diff --git a/test/functional/feature_config_args.py b/test/functional/feature_config_args.py
index 2b93e3c24d7..ed29c3c7af5 100755
--- a/test/functional/feature_config_args.py
+++ b/test/functional/feature_config_args.py
@@ -29,6 +29,11 @@ class ConfArgsTest(BitcoinTestFramework):
             conf.write("wallet=foo\n")
         self.nodes[0].assert_start_raises_init_error(expected_msg='Error: Config setting for -wallet only applied on regtest network when in [regtest] section.')
 
+        with open(inc_conf_file_path, 'w', encoding='utf-8') as conf:
+            conf.write('regtest=0\n') # mainnet
+            conf.write('acceptnonstdtxn=1\n')
+        self.nodes[0].assert_start_raises_init_error(expected_msg='Error: acceptnonstdtxn is not currently supported for main chain')
+
         with open(inc_conf_file_path, 'w', encoding='utf-8') as conf:
             conf.write('nono\n')
         self.nodes[0].assert_start_raises_init_error(expected_msg='Error reading configuration file: parse error on line 1: nono, if you intended to specify a negated option, use nono=1 instead')